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Cell Viability Assays: Advancing Research in Drug Discovery and Cellular Analysis
Cell viability assays are essential tools in biological research, drug discovery, and toxicology, enabling scientists to assess the health, proliferation, and metabolic activity of cells in vitro. These assays provide critical data for understanding cytotoxicity, drug efficacy, and cellular responses to various stimuli.
Overview and Dynamics
Common techniques include MTT, XTT, resazurin reduction, ATP quantification, and flow cytometry-based assays, each offering specific advantages in sensitivity, throughput, and compatibility with different cell types. The choice of assay depends on experimental objectives, cell type, and desired readout.
Cell viability assays are integral in pharmaceutical R&D, allowing early identification of cytotoxic compounds and optimizing therapeutic candidates. Automation, high-throughput screening, and integration with robotics and imaging platforms have improved reproducibility, efficiency, and scalability. Research also focuses on 3D cell cultures, organoids, and co-culture systems, which better mimic in vivo environments, providing more predictive results.












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